Correlation of Antiplasmin to Antitrypsin and Antichymotrypsin in Human Plasma.

The numbers of antiplasmin, antitrypsin and antichymotrypsin in human plasma and mutual relationship among them are discussed in this paper. Human blood plasma was divided into 2 major fractions by adding ammo nium sulfate, after desalting by sephadex, the fractions being further subfrac tionated by using column chromatographic procedure on DEAE-cellulose. The determination of antiproteolytic activities and electrophoretic analysis were performed on the subfractions eluated by the following order of solvents; (1) pH 7.8, 0.0175 M potassium phosphate buffer (KPB), (2) pH 7.1, 0.04 M KPB, (3) pH 6.64, 0.1 M KPB and (4) 0.0025 M hydrochloric acid containing 1 M sodium chloride. Three chromatographically different antiplasmin fractions which were capable of both antitrypsin and antichmyotrypsin activities, and another antitrypsin fraction which showed antichymotrpysin, but was devoid of an tiplasmin activity were detected in human plasma.